Yeast Glutathione Reductase

Glutathione reductase from bakers' yeast and beef liver.

The reduction of glutathione by a heat-labile system in liver was discovered by Hopkins and Elliott (1). Later Mann (2) obtained a soluble enzyme preparation from liver which required, for the reduction process, a cofactor and glucose as hydrogen donor. Meldrum and Tarr (3) found that oxidized glutathione was reduced by rat blood and by yeast and demonstrated the function of TPNl as a cofactor ...

On the reaction mechanism of yeast glutathione reductase.

Glutathione reductase has been purified from a number of sources, Escherichiu coli (l), yeast (2, 3), peas (4), rat tissues (5, 6), and erythrocytes (7). In all of the cases, the enzyme appears to be a flavoprotein, containing flavin adenine dinucleotide as prosthetic group (8). In view of the fact that the over-all reaction catalyzed by the enzyme, GSSG + TPNH + H+ & 2 GSH + TPN+, is formally ...

Mammalian Selenoprotein Thioredoxin-glutathione Reductase

Yeast glutathione reductase. I. Spectrophotometric and kinetic studies of its interaction with reduced nicotinamide adenine dinucleotide.

The interaction of the flavoprotein glutathione reductase from yeast with NADH has been studied. A reduced enzyme species resulting from addition of 2 electrons per enzymebound FAD is formed on addition of NADH to oxidized enzyme. This Z-electron reduced enzyme is stable toward further reduction to the 4-electron reduced state by excess NADH. Yeast glutathione reductase reduced by excess NADH h...

Molecular basis of glutathione reductase deficiency in human blood cells Short title: Human glutathione reductase deficiency

NMK performed research, collected and analyzed data and wrote the paper; RvZ collected patient material, performed research and analyzed data; MdB performed sequence analysis and analyzed data; GM performed sequence analysis; HV performed patient RBC analysis; NB treated one patient and collected data; CL treated one patient, collected data and wrote a clinical report; KMD interviewed three pat...